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محلول نمک فسفات با خاصیت بافری
The buffer was changed to phosphate buffered saline (PBS) to collect the main fraction, which was diluted with a solvent into an appropriate concentration and then used in the following tests.
[00292] In the following tests, 3% BSA/PBS was used as a solvent for adjusting the concentration of an antibody, without otherwise specified.
[00294] A non-phosphorylated tau peptide PD17(Ser413) and a phosphorylated tau peptide PD17P(pSer413), in which Ser413 was phosphorylated, were each diluted with cooled PBS to 1 �g/mL, and the resultant solutions were each dispensed to a plate at 50 �L/well and allowed to stand at 4�C overnight.
The solution was removed, and a blocking buffer (3% bovine serum albumin (BSA)-PBS) was then dispensed at 270 �L/well, and the plate was allowed to stand at 4�C overnight.
After the solution was removed, a blocking buffer (3% bovine serum albumin (BSA)-PBS) was dispensed at 270 ?
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